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2-Deoxy-D-glucose (2-DG)

貨號(hào) ICE1201 售價(jià)(元) 567
規(guī)格 1g CAS號(hào) 154-17-6
  • 產(chǎn)品簡(jiǎn)介
  • 相關(guān)產(chǎn)品

產(chǎn)品信息

貨號(hào)

產(chǎn)品名稱

規(guī)格

價(jià)格/

ICE1201-0001G

2-Deoxy-D-glucose 2-脫氧-D-葡萄糖

1g

567

ICE1201-0005G

2-Deoxy-D-glucose 2-脫氧-D-葡萄糖

5g

2298

ICE1201-0025G

2-Deoxy-D-glucose 2-脫氧-D-葡萄糖

25g

詢價(jià)

產(chǎn)品簡(jiǎn)介

2-脫氧-D-葡萄糖(2-Deoxy-D-glucose, 2-DG),一種不可代謝的葡萄糖類似物,通過(guò)作用于己糖激酶(糖酵解的限速步驟)來(lái)抑制糖酵解。2-DG被己糖激酶磷酸化生成2-DG-P,后者不能被磷酸葡萄糖異構(gòu)酶進(jìn)一步代謝,進(jìn)而引起2-DG-P在細(xì)胞內(nèi)的累積和細(xì)胞內(nèi)ATP的耗盡。體外缺氧模型中,2-DG導(dǎo)致細(xì)胞周期抑制和細(xì)胞死亡,誘導(dǎo)自噬,增加ROS生成和活化AMPK。體內(nèi)動(dòng)物模型中阻斷腫瘤細(xì)胞生長(zhǎng)。

產(chǎn)品特性

1) CAS NO154-17-6

2) 化學(xué)名:(4R,5S,6R)-6-(Hydroxymethyl)oxane-2,4,5-triol

3) 同義名:2-deoxyglucose; 2-Deoxy-D-arabinohexose; D-Arabino-2-deoxyhexose; Ba 2758; NSC 15193; 2-脫氧葡萄糖;2-去氧-D-葡萄糖;D-2-脫氧葡萄糖;

4) 分子式:C6H12O5

5) 分子量:164.16

6) 外觀:白色至類白色固體

7) 純度:>98%

8) 溶解性:溶于DMSO20mg/ml)、H2O30mg/ml)、PBS, pH 7.2 10mg/ml

保存與運(yùn)輸方法

保存:2-8℃避光干燥保存,至少2年有效。 

運(yùn)輸:室溫運(yùn)輸。

注意事項(xiàng)

1) 本品儲(chǔ)存液置于≤-20℃避光保存,盡量避免反復(fù)凍融。通常情況下,-20℃至少一個(gè)月穩(wěn)定,-80℃至少6個(gè)月穩(wěn)定。

2) 本品僅用作科研用途,絕不可用于臨床診斷或治療藥物,絕不可用做食品或藥品。

3)為了您的安全和健康,請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。

儲(chǔ)存液配制

質(zhì)量  濃度

1mg

5mg

10mg

1mM

6.0916 mL

30.4581 mL

60.9162mL

5mM

1.2183mL

6.0916 mL

12.1832mL

10mM

0.6092 mL

3.0458 mL

6.0916 mL

50mM

0.1218 mL

0.6092 mL

1.2183 mL

使用方法【源自文獻(xiàn),僅作參考】

文獻(xiàn)1,Yan J et al. The bifunctional autophagic flux by 2-deoxyglucose to control survival or growth of prostate cancer cells. Cancer Res. 2005 Aug 1;65(15):7023-30. PMID: 16061689

體外研究(細(xì)胞實(shí)驗(yàn)):

細(xì)胞類型(Cell types):MCF-7 cells

實(shí)驗(yàn)方法(Assay):For cell growth assay, MCF-7 cells were plated at 3 × 104 cells per well in flat-bottomed 96-well plates in 100 μL of culture medium. After 24 hours, cells were fed with fresh medium including 2-DG at doses of 0, 4, 8, or 16 mmol/L. At days 1, 3, and 5 after 2-DG exposure, cells were fixed with 1% glutaraldehyde, replaced with PBS, and stored at 4°C. At the end of an experiment, all of the plates were stained with 0.02% aqueous crystal violet for 30 minutes and rinsed with deionized water. After redissolving the bound crystal violet in 70% ethanol, the absorbance was determined at 590 nm. 

實(shí)驗(yàn)結(jié)果(Result):Cell growth was significantly inhibited by 2-DG at doses of 4, 8, or 16 mmol/L for 1, 3, or 5 days in a dose- and time-dependent manner (P < 0.001).

體內(nèi)研究(動(dòng)物模型):

動(dòng)物模型(Animal Model):At 21 days of age, rats were injected with 50 mg 1-methyl-1-nitrosourea per kilogram of body weight (i.p.). Six days following carcinogen injection, all rats were randomized into one of three groups, 30 rats per group, and werefed ad libitum AIN-93G diet containing0.0%, 0.02%, or 0.03% (w/w) 2-DGfor 5 weeks.

實(shí)驗(yàn)結(jié)果(Result):2-DG decreased the incidence and multiplicity of mammary carcinomas and prolonged cancer latency (P < 0.05). The 0.02% dose of 2-DG had no effect on circulating levels of glucose, insulin, insulin-like growth factor-I, IGF binding protein-3, leptin, or body weight gain.

文獻(xiàn)2,Wang H et al. 2-deoxy-D-glucose enhances anesthetic effects in mice. Anesth Analg. 2015 Feb; 120(2):312-9. PMID: 25390277

體內(nèi)研究(動(dòng)物模型):

動(dòng)物模型(Animal Model):Female Sprague-Dawley rats at 20 days of age

配制方法(Formulation):2-DGwasdiluted insaline. 

實(shí)驗(yàn)方法(Assay):2-DG (1000 mg/kg), 3-NP (50 mg/kg),30 or saline was injected [intraperitoneally (ip)] 5 minutes before isoflurane anesthesia.Idebenone (200 mg/kg) or L-carnitine (100 mg/kg) was injected 15 minutes before isoflurane anesthesia (10 minutes before treatment with 2-DG or 3-NP).

實(shí)驗(yàn)結(jié)果(Result):2-DG enhances isoflurane-induced loss of righting reflex (LORR) in mice.



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