產(chǎn)品信息

產(chǎn)品簡介

       CopyCutter? EPI400? Electrocompetent and Chemically Competent E. coli cells were developed to significantly lower the copy number of a wide  variety of common vectors so that you can more readily clone unstable DNA sequences. Moreover, following a short incubation in the   presence of the CopyCutter Induction Solution, you can subsequently raise copy number to   improve plasmid yields without compromising stability.

       CopyCutter?EPI400?電感受態(tài)和化學(xué)感受態(tài)大腸桿菌細胞的開發(fā)可顯著降低各種常見載體的拷貝數(shù)，因此您可以更輕松地克隆不穩(wěn)定的DNA序列。此外，在存在CopyCutter誘導(dǎo)溶液的情況下進行短暫孵育后，您可以隨后增加拷貝數(shù)以提高質(zhì)粒產(chǎn)量，而不會影響穩(wěn)定性。

        The CopyCutter EPI400 cell line was derived from our high-transformation efficiency phage T1-resistant TransforMax? EC100? E. coli strain by manipulating a gene that controls the copy number of vectors containing ColE1 or pMB1 origins of replication (e.g., pUC and pET type   vectors). This constitutively expressed gene, pcnB (plasmid copy number), was deleted from the TransforMax EC100 strain and replaced with a modified pcnB gene linked to an inducible   promoter, creating the CopyCutter EPI400 strain.

         通過控制含有ColE1或pMB1復(fù)制起點（例如pUC和pET類型）的載體的拷貝數(shù)的基因，CopyCutter EPI400細胞系源自我們的高轉(zhuǎn)化效率噬菌體T1抗性TransforMax?EC100?大腸桿菌菌株向量)。TransforMax EC100菌株中刪除了這個組成性表達的基因pcnB（質(zhì)?？截悢?shù)），并用與誘導(dǎo)型啟動子連接的修飾的pcnB基因代替，從而創(chuàng)建了CopyCutter EPI400菌株。

優(yōu)點：

        High transformation efficiency with clones of all sizes.

        Supports blue/white screening of vectors.

        Restriction minus [mcrA ?(mrr-hsdRMS-mcrBC)] for efficient cloning of methylated DNA.

        Endonuclease minus (endA1) to ensure high yields of plasmid clones.

        Recombination minus (recA1) to ensure the stability of large cloned inserts.

 技術(shù)參數(shù)

產(chǎn)品優(yōu)點- High transformation efficiency with clones of all sizes.

- Supports blue/white screening of vectors.

- Restriction minus [mcrA ?(mrr-hsdRMS-mcrBC)] for efficient cloning of methylated DNA.

- Endonuclease minus (endA1) to ensure high yields of plasmid clones.

- Recombination minus (recA1) to ensure the stability of large cloned inserts.

產(chǎn)品應(yīng)用- Stabilize toxic inserts in common cloning and expression vectors (pUC and pET-type vectors)

- Clone and maintain challenging sequences at reduced plasmid copy number, then induce to high copy number for DNA recovery

- Avoid T1 and T5 phage contamination with tonA mutation

- Choose chemically competent cells for general cloning or electrocompetent cells for demanding applications such as library generation