產(chǎn)品信息

SPECIFICITY：

Degenerating neurons, and neuronal degeneration. There is no specific staining in normal healthy brain. Note: Some researchers under some conditions report blood vessel staining with Fluoro Jade. This may be because Fluoro Jade is an analogue of eosin (which stains blood cells).In general, good perfusion and preparation of the tissue should help prevent blood vessel staining but it may not be possible to eliminate it entirely. In our experience it is generally possible to distinguish neuronal from blood vessels staining by eye.

APPLICATION(S) ：FC, ICC

APPLICATION DETAILS：

Following our detailed protocol, Fluoro-Jade C labels degenerating neurons which are visualised with blue light excitation, while DAPI (not included) counter stains cell nuclei, visualised with ultra-violet illumination. The Fluoro-Jade C dye can be used on all kinds of preserved tissues, including fresh-frozen, paraformaldehyde or formalin fixed, and formalin fixed, paraffinembedded tissues.

TARGET ：Degenerating neurons

ALTERNATIVE NAMES ：FJB, Fluoro-Jade

TARGET HOST SPECIES :Species Independent

SPECIES REACTIVITY :Human, Mouse, Other Mammals (Predicted), Rat

DETECTION METHOD :Fluorescence

EX/EM MAX :

      FJB visualization is accomplished using blue light or a 488 nm Laser.

      Excitation Peak: 495 nm

      Emission Peak: 521 nm

      Filter system for visualizing: Fluorescein/FITC

KIT COMPONENTS :

Materials Provided:

      30 mg Fluoro-Jade B, dry powder

      Detailed protocol

Equipment and Reagents Required:

      Distilled water

      ACS grade Ethanol (200 proof) for slide & solution preparation

      1% sodium hydroxide in 80% ethanol (basic alcohol solution)

      0.1% Acetic Acid solution (in water)

      70% ethanol in distilled water

      0.06% (KMnO4) potassium permanganate solution

      DAPI powder or 100X solution (working range is 0.5-5 μg/mL)

      Xylene liquid

      Staining dishes/Coplin jars

      Cover slips

      DPX mounting media or another permanent mounting medium. Non-polar media are preferredover aqueous mounting media such as glycerin/water to obtain high- contrast images (refer to Appendix B in the protocol for a comparative analysis). Traditional fluorescent mounting mediums are not recommended because of their high pH.

      Slide warmer

      Convection oven

FORMAT :

Dry, Coffee brown to brick red powder; hygroscopic powder keep dessicated.

RECONSTITUTION INSTRUCTIONS :

Spin vial briefly before opening. See product protocol for detailed use instructions.

PURITY DESCRIPTION :

Thin layer chromatograpy using cellulose plates and a solvent system of n-propinol, water, and ammonium hydroxide (6:5:2) revealed the presence of two fluorescent isomers and two trace non-fluorescent bands. No amount of fluorescein or Fluoro-Jade was present.

STORAGE INSTRUCTIONS :

The powdered dye can be stored desiccated at room temperature in the dark. Storage in a desiccator is recommended as FJB is hydroscopic. The 0.01% stock solution will remain stable for 3 months when stored in a refrigerator, in the dark. The 0.0001-0.0004% working solution in 0.1% acetic acid should be used within 4 hours of preparation. Diluted FJB dye solutions are not stable and should not be stored. The other diluted solutions can be reused and stored for up to 48 hours if refrigerated and protected from light. Best results require freshly diluted solutions.

EXPIRATION DATE: 

6 months after date of receipt (unopened vial).

ADDITIONAL IMAGES STATEMENT: 

Please refer to the Biosensis website for additional product images.

REFERENCES STATEMENT: 

Please refer to our website for product-specific references.

REGULATORY STATUS :For research use only

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